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Anal Biochem. 1988 Dec;175(2):525-30.

Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.

Author information

1
Laboratoire de Biochimie Cellulaire et Moléculaire des Glycoconjugués, Centre National de la Recherche Scientifique, Orleans, France.

Abstract

A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.

PMID:
3239778
DOI:
10.1016/0003-2697(88)90578-7
[Indexed for MEDLINE]

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