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Prenat Diagn. 1988 Sep;8(7):485-93.

Prenatal diagnosis with biotinylated chromosome specific probes.

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Centre de Diagnostic Prenatal et de Foetologie, Institut de Puériculture, Paris, France.


We have used a Y-chromosome specific DNA probe in a controlled study to determine the presence of Y-chromosome material and to detect numerical abnormalities in uncultured amniotic fluid cells by fluorescent hybridization. Using this non-radioactive method, we correctly predicted fetal sex within 48 h in all but 3 of 54 cases and identified an XYY syndrome. The technique was previously tested with no false-positive or false-negative results on cultured interphase or metaphase nuclei of fetal fibroblasts and adult T-lymphocytes. Fluorescent in situ hybridization was applied to long-term fixed cytogenetic preparations up to 44 months old and was shown to be reliable.

[Indexed for MEDLINE]

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