Neutrophil-derived exosome from systemic sclerosis inhibits the proliferation and migration of endothelial cells

Biochem Biophys Res Commun. 2020 May 28;526(2):334-340. doi: 10.1016/j.bbrc.2020.03.088. Epub 2020 Mar 24.

Abstract

Objectives: Systemic sclerosis (SSc) is an autoimmune disease characterized by vasculopathy, inflammation, and extensive fibrosis in multiple organs. Exosomes (EXOs) are cell-derived vesicles contained various DNAs, RNAs and proteins, and play important roles in various diseases. Here, we aimed to investigate the roles of SSc EXOs in angiogenesis related mechanisms.

Methods: EXOs were isolated from plasma, cultured peripheral blood mononuclear cells (PBMCs)/neutrophil supernatants, and identified by transmission electron microscopy. The expression of S100A8/A9 was measured by real-time PCR and ELISA. Proliferation, migration and scratch assays in human dermal microvascular endothelial cells (HDMECs) were used to study the EXOs influence.

Results: Plasma and neutrophil EXOs from SSc patients can suppress the proliferation and migration of HDMECs. High levels of S100A8/A9 were found in SSc EXOs which derived from plasma, PBMCs and neutrophils. The expression of S100A8/A9 in neutrophil EXOs was higher than that in PBMC EXOs in SSc patients. The proliferation and migration of HDMECs were possibly inhibited by S100A8/A9 of neutrophil EXOs.

Conclusions: Neutrophil EXOs from SSc patients inhibits the proliferation and migration of HDMECs, S100A8/A9 might play an important role in this process.

Keywords: Exosome; Human dermal microvascular endothelial cell; Neutrophil; S100A8/A9; Systemic sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Movement
  • Cell Proliferation
  • Endothelial Cells / cytology
  • Endothelial Cells / pathology*
  • Exosomes / pathology*
  • Female
  • Humans
  • Male
  • Neutrophils / cytology
  • Neutrophils / pathology*
  • Scleroderma, Systemic / pathology*