Aspalathin-rich green Aspalathus linearis extract suppresses migration and invasion of human castration-resistant prostate cancer cells via inhibition of YAP signaling

Phytomedicine. 2020 Apr:69:153210. doi: 10.1016/j.phymed.2020.153210. Epub 2020 Mar 18.

Abstract

Background: More than 80% of advanced prostate cancer (PCa) cases have bone metastasis, with a 5-year survival rate of 25%. Previously, we reported that GRT, a standardized, pharmaceutical-grade aspalathin-rich extract (12.78 g aspalathin/100 g extract), prepared from green rooibos produced from the leaves and fine stems of Aspalathus linearis, inhibits the proliferation of PCa cells, meriting this investigation to determine if GRT can suppress the migration and invasion of castration-resistant prostate cancer (CRPC) cells.

Purpose: In the present study, we investigated whether GRT extract can interfere with the migration and invasion of human CRPC cells.

Methods: Transwell assays were used to explore the effects of GRT on the migration and invasion of CRPC cells. Micro-Western Array (MWA) and Western blot analysis were carried out to unravel the underlying molecular mechanism(s).

Results: Treatment with 25-100 μg/ml GRT suppressed the migration and invasion of LNCaP C4-2B and 22Rv1 CRPC cells. MWA and Western blot analysis indicated that GRT treatment suppressed the protein level of yes-associated protein (YAP), macrophage stimulating 1 protein (MST1), phospho-MST1/phospho-MST2 T183/T180, and paxillin, but increased the abundance of E-cadherin. Over-expression of YAP rescued the suppressive effects of GRT on migration and invasion of CRPC cells. Treatment with the major flavonoid of GRT - the C-glucosyl dihydrochalcone, aspalathin - at a concentration of 75-100 μg/ml also reduced the migration and invasion of CRPC cells, and the inhibition was partially rescued by YAP over-expression.

Conclusions: GRT treatment suppresses the migration and invasion of CRPC cells via inhibition of YAP signaling and paxillin.

Keywords: Aspalathin; Invasion; Migration; Prostate cancer; Rooibos; YAP.

MeSH terms

  • Adaptor Proteins, Signal Transducing / antagonists & inhibitors
  • Adaptor Proteins, Signal Transducing / metabolism
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Aspalathus / chemistry*
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Chalcones / pharmacology*
  • Humans
  • Male
  • Paxillin / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Prostatic Neoplasms, Castration-Resistant / drug therapy*
  • Prostatic Neoplasms, Castration-Resistant / pathology
  • Transcription Factors / antagonists & inhibitors
  • Transcription Factors / metabolism
  • YAP-Signaling Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • Antineoplastic Agents, Phytogenic
  • Chalcones
  • PXN protein, human
  • Paxillin
  • Plant Extracts
  • Transcription Factors
  • YAP-Signaling Proteins
  • YAP1 protein, human
  • aspalathin