Generation of twelve induced pluripotent stem cell lines from two healthy controls and two patients with sporadic amyotrophic lateral sclerosis

Meimei Yang; Min Liu; Yicheng Ding; Alice Vajda; Jun Ma; Huixian Cui; Timothy O'Brien; David Henshall; Orla Hardiman; Sanbing Shen

doi:10.1016/j.scr.2020.101752

Generation of twelve induced pluripotent stem cell lines from two healthy controls and two patients with sporadic amyotrophic lateral sclerosis

Stem Cell Res. 2020 Apr:44:101752. doi: 10.1016/j.scr.2020.101752. Epub 2020 Mar 17.

Authors

Meimei Yang¹, Min Liu², Yicheng Ding³, Alice Vajda⁴, Jun Ma⁵, Huixian Cui⁵, Timothy O'Brien⁶, David Henshall⁷, Orla Hardiman⁸, Sanbing Shen⁹

Affiliations

¹ Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland.
² Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
³ Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland.
⁴ Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland.
⁵ Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Research Center for Stem Cell Medical Translational Engineering, Human Anatomy Department, Hebei Medical University, Hebei 050017, China.
⁶ Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China.
⁷ FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland.
⁸ FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland; Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland. Electronic address: orla@hardiman.net.
⁹ Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland; Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China. Electronic address: Sanbing.Shen@nuigalway.ie.

PMID: 32208303
DOI: 10.1016/j.scr.2020.101752

Abstract

The majority of amyotrophic lateral sclerosis are sporadic (sALS) with no familial history or known genetic association, therefore a large cohort of disease models are required to identify common mechanisms or to test therapeutic interventions. Here we generated twelve induced pluripotent stem cell (iPSC) lines from human dermal fibroblasts of two healthy individuals and two sALS patients lacking common ALS mutations, using non-integrational Sendai virus expressing reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC. The iPSC lines highly expressed pluripotency markers could be spontaneously differentiated into three embryonic germ layers, with no gross chromosomal aberrations or specific copy number variations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyotrophic Lateral Sclerosis* / genetics
Cell Differentiation
DNA Copy Number Variations
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4

Substances

KLF4 protein, human
Kruppel-Like Factor 4