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Sci Total Environ. 2020 Mar 7;722:137795. doi: 10.1016/j.scitotenv.2020.137795. [Epub ahead of print]

Application of molecular techniques in biohydrogen production as a clean fuel.

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School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea; Department of Chemistry, Bioscience and Environmental Engineering, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway.
Department of Energy and Environment, National Institute of Technology, Tiruchirappalli - 620015, Tamil Nadu, India.
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044 China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Department of Biological Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam. Electronic address:


Considering the future energy demand and pollution to the environment, biohydrogen, a biofuel, produced from biological sources have garnered increased attention. The present review emphasis the various techniques and methods employed to enumerate the microbial community and enhancement of hydrogen production by dark fermentation. Notably, molecular techniques such as terminal restriction fragment length polymorphism (T-RFLP), quantitative real-time PCR (q-PCR), fluorescent in-situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), ribosomal intergenic spacer analysis (RISA), and next generation sequencing (NGS) have been extensively discussed on identifying the microbial population in hydrogen production. Further, challenges and merits of the molecular techniques have been elaborated.


Biohydrogen; DGGE; Energy; FISH; Molecular techniques

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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