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J Econ Entomol. 2020 Mar 24. pii: toaa043. doi: 10.1093/jee/toaa043. [Epub ahead of print]

Detection of Wolbachia (Rickettsiales: Anaplasmataceae) and Candidatus Liberibacter asiaticus (Rhizobiales: Rhizobiaceae) Associated With Diaphorina citri (Hemiptera: Liviidae) Collected From Citrus reticulata (Sapindales: Rutaceae) and Alternate Host, Cordia myxa (Boraginales: Boraginaceae).

Author information

1
Molecular Entomology Lab, Department of Plant Protection, Faculty of Agriculture, Ankara University, Dıskapi, Ankara, Turkey.
2
Department of Entomology, University of Sargodha, Sargodha, Pakistan.
3
Department of Molecular Biology and Genetics, Necmettin Erbakan University, Meram, Konya, Turkey.

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important insect pest of the citrus crop worldwide. It vectors the pathogen 'Candidatus Liberibacter asiaticus' (CLas) that causes a serious disease known as citrus greening. Here, we tested the infection frequency of Wolbachia and CLas from 100 D. citri individuals collected from two host plants belonging to families Rutaceae (Citrus reticulata Blanco) and Boraginaceae (Cordia myxa L.) using molecular methods. The following trend of endosymbionts infection in adult D. citri was found; 85.4% (35/41) by Wolbachia, and 19.5% (8/41) by CLas collected from C. reticulata plants and 65.4% (17/26) by Wolbachia, and 15.4% (4/26) by CLas in case of C. myxa plant. However, 61.5% (8/13) nymphs collected from C. reticulata and 20.0% (4/20) collected from C. myxa plants were infected by Wolbachia, while no nymph was infected by CLas collected from either host plants. Findings from this work represent the first report of CLas presence in D. citri feeding on C. myxa plants. By studying the presence of CLas with other endosymbiotic bacteria, future basic and applied research to develop control strategies can be prioritized.

KEYWORDS:

Citrus reticulata; Cordia myxa; Diaphorina citri; endosymbionts; ‘Candidatus Liberibacter asiaticus’

PMID:
32207826
DOI:
10.1093/jee/toaa043

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