Format

Send to

Choose Destination
Heart Vessels. 2020 Mar 23. doi: 10.1007/s00380-020-01582-4. [Epub ahead of print]

Elevated chymase-dependent angiotensin II-forming activity in circulating mononuclear leukocytes was observed in the patient of atrial fibrillation.

Author information

1
Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, 1-1-1, Zokumyoin, Chikushino-shi, Fukuoka, 818-8502, Japan. okamurakmd@cis.fukuoka-u.ac.jp.
2
Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, 1-1-1, Zokumyoin, Chikushino-shi, Fukuoka, 818-8502, Japan.

Abstract

Chymase is an angiotensin II-forming serine proteinase and elevation of its tissue activity occurs in various cardiovascular diseases. Several authors have suggested that there is an association between the renin-angiotensin system and atrial fibrillation (AF). Chymase-dependent angiotensin II-forming activity in circulating mononuclear leukocytes (CML chymase dAIIFA) was investigated in patients with AF and patients in sinus rhythm. Consecutive outpatients were recruited at our hospital. CML chymase dAIIFA was measured using a Nma/Dnp-type fluorescence-quenching substrate of modified angiotensin I in the presence or absence of a specific serine proteinase inhibitor. To search the independent contributing factor of existence of AF, the analysis between groups was carried out using multivariate analysis after univariate analysis. The patients were classified into a sinus rhythm (SR) group (n = 459) or an AF group (n = 48). CML chymase dAIIFA was significantly higher in the AF group (622 pmol/min/mg) compared with the SR group (488 pmol/min/mg) (p < 0.001). Logistic regression analysis revealed that high CML chymase dAIIFA was an independent determinant of the existence of AF (p < 0.001). Elevation of CML chymase dAIIFA was associated with AF. Activation of chymase might be linked to atrial structural and electrical remodeling.

KEYWORDS:

Angiotensin II-forming enzyme activity; Atrial fibrillation; Chymase; Circulating mononuclear leukocyte; Human; Serine proteinase

PMID:
32206865
DOI:
10.1007/s00380-020-01582-4

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center