Format

Send to

Choose Destination
FASEB J. 2020 Mar 23. doi: 10.1096/fj.201903049R. [Epub ahead of print]

Stimulation of water and calcium dynamics in astrocytes with pulsed infrared light.

Author information

1
Istituto per lo Studio dei Materiali Nanostrutturati, Consiglio Nazionale delle Ricerche, Bologna, Italy.
2
Department Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
3
Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, USA.
4
Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Bologna, Italy.
5
Department of Bioscience, Biotechnology and Biopharmaceutics and Centre of Excellence in Comparative Genomics, University of Bari Aldo Moro, Bari, Italy.
6
Bioengineering College, Chongqing University, Chongqing, China.
7
Dipartimento di Farmacia e Biotecnologie, University of Bologna, Bologna, Italy.
8
Department of Basic Medical Sciences, Neuroscience and Sense Organs, School of Medicine, University of Bari Aldo Moro, Bari, Italy.
9
Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, New York, NY, USA.
10
Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, Adelaide Medical School, University of Adelaide, Adelaide, SA, Australia.
11
Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.

Abstract

Astrocytes are non-neuronal cells that govern the homeostatic regulation of the brain through ions and water transport, and Ca2+ -mediated signaling. As they are tightly integrated into neural networks, label-free tools that can modulate cell function are needed to evaluate the role of astrocytes in brain physiology and dysfunction. Using live-cell fluorescence imaging, pharmacology, electrophysiology, and genetic manipulation, we show that pulsed infrared light can modulate astrocyte function through changes in intracellular Ca2+ and water dynamics, providing unique mechanistic insight into the effect of pulsed infrared laser light on astroglial cells. Water transport is activated and, IP3 R, TRPA1, TRPV4, and Aquaporin-4 are all involved in shaping the dynamics of infrared pulse-evoked intracellular calcium signal. These results demonstrate that astrocyte function can be modulated with infrared light. We expect that targeted control over calcium dynamics and water transport will help to study the crucial role of astrocytes in edema, ischemia, glioma progression, stroke, and epilepsy.

KEYWORDS:

AQP4; TRPV4; astrocytes; infrared stimulation; water dynamics

PMID:
32202681
DOI:
10.1096/fj.201903049R

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center