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New Phytol. 2020 Mar 15. doi: 10.1111/nph.16546. [Epub ahead of print]

Mutations in the predicted DNA polymerase subunit POLD3 result in more rapid flowering of Brachypodium distachyon.

Author information

1
Laboratory of Genetics, University of Wisconsin, 425-G Henry Mall, Madison, WI, 53706.
2
United States Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison.
3
Department of Biochemistry, University of Wisconsin, 433 Babcock Dr. Madison, Madison, WI, 53706.
4
Plant Sciences Department, University of California-Davis, One Shields Ave, 104 Robbins Hall, Davis, CA, 95616.
5
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
6
Department of Plant Biology and Department of Molecular Biology and Biochemistry, Michigan State University, East Lansing, MI, 48824, United States.
7
United States Department of Energy Joint Genome Institute, Berkeley, California, 94720.
8
INRAE, UR BIA, F-44316, Nantes, France.
9
Institut Jean-Pierre Bourgin, UMR 1318, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France.
10
USDA-ARS Western Regional Research Center.
11
Lawrence Berkeley National Laboratory, Berkeley, CA, 94720.
12
University of California Berkeley, Berkeley, CA, 94704.

Abstract

The timing of reproduction is a critical developmental decision in the life cycle of many plant species. Fine mapping of a rapid-flowering mutant was done using whole-genome sequence data from bulked DNA from a segregating F2 mapping populations. The causative mutation maps to a gene orthologous with the third subunit of DNA polymerase δ (POLD3), a previously uncharacterized gene in plants. Expression analyses of POLD3 were conducted via real time qPCR to determine when and in what tissues the gene is expressed. To better understand the molecular basis of the rapid-flowering phenotype, transcriptomic analyses were conducted in the mutant versus wild type. Consistent with the rapid-flowering mutant phenotype, a range of genes involved in floral induction and flower development are upregulated in the mutant. Our results provide the first characterization of the developmental and gene expression phenotypes that result from a lesion in POLD3 in plants.

KEYWORDS:

Brachypodium distachyon ; DNA polymerase; POLD3; flowering; grasses

PMID:
32173866
DOI:
10.1111/nph.16546

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