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Methods Mol Biol. 2020;2123:393-400. doi: 10.1007/978-1-0716-0346-8_30.

Equine Influenza Culture Methods.

Author information

1
Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. tmcham1@uky.edu.
2
Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.

Abstract

Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

KEYWORDS:

Embryonated eggs; MDCK; Mammalian influenza; Plaque assay; TCID assay

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