Myelin formation in rat dorsal root ganglion cultured in a serum-free medium: influence of various culture media on myelin formation

Neurosci Res. 1988 Dec;6(2):115-22. doi: 10.1016/0168-0102(88)90013-2.

Abstract

Survival and myelination in dorsal root ganglion culture were observed using 6 kinds of modified commercial serum-free media: MEM, neMEM, alpha-MEM, DMEM, F12 medium and DF medium (see Materials and Methods). The results showed that the survival rates of dorsal root ganglia cultured in alpha-MEM and DF medium are the highest and that the rates of myelinated ganglia are the highest when alpha-MEM is used as culture medium, as compared with other serum-free media.

MeSH terms

  • Animals
  • Cell Survival
  • Cells, Cultured
  • Culture Media / pharmacology*
  • Embryo, Mammalian
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / physiology*
  • Myelin Sheath / physiology*
  • Nerve Fibers, Myelinated / drug effects
  • Nerve Fibers, Myelinated / physiology*
  • Rats
  • Rats, Inbred Strains
  • Time Factors

Substances

  • Culture Media