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Nat Plants. 2020 Mar;6(3):314-320. doi: 10.1038/s41477-020-0610-x. Epub 2020 Mar 9.

Distinct structural modulation of photosystem I and lipid environment stabilizes its tetrameric assembly.

Author information

1
Beijing Engineering Research Center for Biofuels, Institute of Nuclear and New, Energy Technology, Tsinghua University, Beijing, P. R. China.
2
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
3
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
4
Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Joint, Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, P. R. China.
5
Beijing Engineering Research Center for Biofuels, Institute of Nuclear and New, Energy Technology, Tsinghua University, Beijing, P. R. China. szli@tsinghua.edu.cn.
6
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden. amunts@scilifelab.se.
7
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. amunts@scilifelab.se.

Abstract

Photosystem I (PSI) is able to form different oligomeric states across various species. To reveal the structural basis for PSI dimerization and tetramerization, we structurally investigated PSI from the cyanobacterium Anabaena. This revealed a disrupted trimerization domain due to lack of the terminal residues of PsaL in the lumen, which resulted in PSI dimers with loose connections between monomers and weaker energy-coupled chlorophylls than in the trimer. At the dimer surface, specific phospholipids, cofactors and interactions in combination facilitated recruitment of another dimer to form a tetramer. Taken together, the relaxed luminal connections and lipid specificity at the dimer interface account for membrane curvature. PSI tetramer assembly appears to increase the surface area of the thylakoid membrane, which would contribute to PSI crowding.

PMID:
32170279
DOI:
10.1038/s41477-020-0610-x

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