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Curr Biol. 2020 Feb 27. pii: S0960-9822(20)30177-9. doi: 10.1016/j.cub.2020.02.004. [Epub ahead of print]

Temporal Sharpening of Sensory Responses by Layer V in the Mouse Primary Somatosensory Cortex.

Author information

1
Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 16163 Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, 16163 Genova and 38068 Rovereto, Italy.
2
Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 16163 Genova, Italy.
3
Neural Coding Laboratory, Istituto Italiano di Tecnologia, 16163 Genova and 38068 Rovereto, Italy.
4
Department of Basic Neurosciences, Geneva University Neurocenter, Faculty of Medicine, University of Geneva, 1206 Geneva, Switzerland.
5
Neural Coding Laboratory, Istituto Italiano di Tecnologia, 16163 Genova and 38068 Rovereto, Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive Systems @UniTn, Istituto Italiano di Tecnologia, 38068 Rovereto, Italy.
6
Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 16163 Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, 16163 Genova and 38068 Rovereto, Italy. Electronic address: tommaso.fellin@iit.it.

Abstract

The timing of stimulus-evoked spikes encodes information about sensory stimuli. Here we studied the neural circuits controlling this process in the mouse primary somatosensory cortex. We found that brief optogenetic activation of layer V pyramidal cells just after whisker deflection modulated the membrane potential of neurons and interrupted their long-latency whisker responses, increasing their accuracy in encoding whisker deflection time. In contrast, optogenetic inhibition of layer V during either passive whisker deflection or active whisking decreased accuracy in encoding stimulus or touch time, respectively. Suppression of layer V pyramidal cells increased reaction times in a texture discrimination task. Moreover, two-color optogenetic experiments revealed that cortical inhibition was efficiently recruited by layer V stimulation and that it mainly involved activation of parvalbumin-positive rather than somatostatin-positive interneurons. Layer V thus performs behaviorally relevant temporal sharpening of sensory responses through circuit-specific recruitment of cortical inhibition.

KEYWORDS:

GABAergic interneurons; cortical layers; parvalbumin; somatosensory cortex; somatostatin; whisking

PMID:
32169206
DOI:
10.1016/j.cub.2020.02.004
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Conflict of interest statement

Declaration of Interests The authors declare no competing interests.

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