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Nat Commun. 2020 Mar 9;11(1):1268. doi: 10.1038/s41467-020-15059-5.

Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.

Author information

1
Department of Medicine III, Klinikum rechts der Isar, Technical University of Munich, 81675, Munich, Germany.
2
TranslaTUM, Center for Translational Cancer Research, Technical University of Munich, 81675, Munich, Germany.
3
German Center for Neurodegenerative Diseases (DZNE), 72076, Tübingen, Germany.
4
Department of Medicine II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
5
Institute of Molecular Oncology and Functional Genomics, Technical University of Munich, Munich, Germany.
6
University of Tübingen, Center for Ophthalmology, Institute for Ophthalmic Research, 72076, Tübingen, Germany.
7
Deutsches Konsortium für Translationale Krebsforschung (DKTK), 69120, Heidelberg, Germany.
8
Theodor Boveri Institute, Department of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, 97074, Würzburg, Germany.
9
Department of Medicine III, Klinikum rechts der Isar, Technical University of Munich, 81675, Munich, Germany. k.clemm@dkfz-heidelberg.de.
10
Deutsches Krebsforschungszentrum (DKFZ), 69120, Heidelberg, Germany. k.clemm@dkfz-heidelberg.de.
11
CellNetworks Cluster of Excellence, University of Heidelberg, 69120, Heidelberg, Germany. k.clemm@dkfz-heidelberg.de.
12
Department of Medicine III, Klinikum rechts der Isar, Technical University of Munich, 81675, Munich, Germany. florian.bassermann@tum.de.
13
TranslaTUM, Center for Translational Cancer Research, Technical University of Munich, 81675, Munich, Germany. florian.bassermann@tum.de.
14
Deutsches Konsortium für Translationale Krebsforschung (DKTK), 69120, Heidelberg, Germany. florian.bassermann@tum.de.

Abstract

Regulation of mitosis secures cellular integrity and its failure critically contributes to the development, maintenance, and treatment resistance of cancer. In yeast, the dual phosphatase Cdc14 controls mitotic progression by antagonizing Cdk1-mediated protein phosphorylation. By contrast, specific mitotic functions of the mammalian Cdc14 orthologue CDC14B have remained largely elusive. Here, we find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival. Starting from an unbiased proteome-wide screening approach, we specify Wilms' tumor protein 1 (WT1) as the relevant substrate that becomes deubiquitylated and stabilized by serine 2563-phosphorylated USP9X in mitosis. We further demonstrate that WT1 functions as a mitotic transcription factor and specify CXCL8/IL-8 as a target gene of WT1 that conveys mitotic survival. Together, we describe a ubiquitin-dependent signaling pathway that directs a mitosis-specific transcription program to regulate mitotic survival.

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