Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

L D Shuvalova; A V Eremeev; A N Bogomazova; E V Novosadova; E A Zerkalenkova; Yu V Olshanskaya; E Y Fedotova; E S Glagoleva; S N Illarioshkin; O S Lebedeva; M A Lagarkova

doi:10.1016/j.scr.2020.101733

Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

Stem Cell Res. 2020 Apr:44:101733. doi: 10.1016/j.scr.2020.101733. Epub 2020 Feb 24.

Authors

Affiliations

¹ Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
² Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia. Electronic address: art-eremeev@yandex.ru.
³ Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
⁴ Russian Academy of Sciences, Institute of Molecular Genetics, Kurchatov Square 2, 123182 Moscow, Russia.
⁵ Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology Oncology and Immunology, 1 Samory Mashela str, 117997 Moscow, Russia.
⁶ Federal Research Centre of Neurology, Russian Academy of Sciences, 80, Volokolamskoye Shosse, 125367 Moscow, Russia.
⁷ Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119991 Moscow, Russia.

PMID: 32151951
DOI: 10.1016/j.scr.2020.101733

Abstract

IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line
Embryoid Bodies
Exons / genetics
Humans
Induced Pluripotent Stem Cells*
Male
Parkinson Disease* / genetics