Parallel multiphoton excited fabrication of tissue engineering scaffolds using a diffractive optical element

Opt Express. 2020 Feb 3;28(3):2744-2757. doi: 10.1364/OE.381362.

Abstract

Multiphoton excited photochemistry is a powerful technique for freeform nano/microfabrication. However, the construction of large and complex structures using single point scanning is slow, where this is a significant limitation for biological investigations. We demonstrate increased throughput via parallel fabrication using a diffractive optical element. To implement an approach with large field of view and near-theoretical resolution, a scan lens was designed that is optimized for using low-magnification high NA objective lenses. We demonstrate that with this approach it is possible to synthesize large scaffolds at speeds several times faster than by single point scanning.

MeSH terms

  • Cell Line, Tumor
  • Computer Simulation
  • Female
  • Humans
  • Lenses
  • Microtechnology*
  • Optical Devices*
  • Ovarian Neoplasms / diagnostic imaging
  • Photons*
  • Tissue Engineering*
  • Tissue Scaffolds / chemistry*