Format

Send to

Choose Destination
Int J Infect Dis. 2020 Feb 20. pii: S1201-9712(20)30087-4. doi: 10.1016/j.ijid.2020.02.029. [Epub ahead of print]

Association of FCN2 polymorphisms and Ficolin-2 levels with dengue fever in Vietnamese patients.

Author information

1
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam; Department of Biology and Medical Genetics, Vietnam Military Medical University, Hanoi, Vietnam.
2
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam. Electronic address: hoangvantong@vmmu.edu.vn.
3
103 Military Hospital, Vietnam Military Medical University, Hanoi, Vietnam.
4
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.
5
Thong Nhat hospital, Ho Chi Minh City, Vietnam.
6
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam.
7
Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Vietnamese-German Center for Medical Research, Hanoi, Vietnam; Duy Tan University, Da Nang, Vietnam.
8
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam. Electronic address: toannl@vmmu.edu.vn.

Abstract

BACKGROUND AND OBJECTIVE:

The human ficolin-2, encoded by FCN2, recognizes pathogen-associated acetylated residues on their cell surfaces and activates the lectin complement cascade. This study aimed to investigate the contribution of human ficolin-2 and the functional FCN2 genetic variants in dengue virus (DENV) infection and in clinical progression.

METHODS:

FCN2 genetic polymorphisms in the promoter, intron 7 and exon 8 were genotyped in 279 patients with dengue fever and in 200 healthy controls by direct Sanger sequencing. The ficolin-2 levels were measured in serum samples by ELISA and correlated with clinical data.

RESULTS:

The frequencies of +6031GG, +6220GG and +6424TT genotypes were significantly higher in dengue patients compared to healthy controls indicating an increased risk of dengue fever. The SNPs rs11103563 (+6031A/G), rs7872508 (+6220 T/G), and rs7851696 (+6424 G/T) significantly regulated ficolin-2 levels in dengue patients (P < 0.0001). Ficolin-2 levels were increased in patients with Dengue Hemorrhagic Fever (DHF) and Dengue with Warning Signs (DWS) compared to healthy controls (P < 0.0001 and P = 0.038, respectively). Ficolin-2 levels were significantly increased after 10-14 days of admission in both DHF and DWS patients and then slightly decreased after three weeks of discharge, indicating that ficolin-2 levels were modulated during the progression of dengue fever. In addition, ficolin-2 levels were negatively correlated with AST levels and positively correlated with platelet counts.

CONCLUSIONS:

FCN2 polymorphisms are associated with dengue fever in the Vietnamese population. Ficolin-2 levels are modulated during the progression of dengue fever and correlated with clinical parameters and thus may play a possible role in the pathogenesis of DENV infection.

KEYWORDS:

DENV infection; Dengue fever; FCN2 polymorphism; Ficolin-2; severe dengue

PMID:
32088336
DOI:
10.1016/j.ijid.2020.02.029
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center