Mass cytometry identifies expansion of double positive and exhausted T cell subsets in the tumour microenvironment of patients with POEMS syndrome

Taxiarchis V Kourelis; Dragan Jevremovic; Erik Jessen; Surendra Dasari; Jose C Villasboas; Angela Dispenzieri; Shaji Kumar

doi:10.1111/bjh.16522

Mass cytometry identifies expansion of double positive and exhausted T cell subsets in the tumour microenvironment of patients with POEMS syndrome

Br J Haematol. 2020 Jul;190(1):79-83. doi: 10.1111/bjh.16522. Epub 2020 Feb 20.

Authors

Taxiarchis V Kourelis¹, Dragan Jevremovic², Erik Jessen³, Surendra Dasari³, Jose C Villasboas¹, Angela Dispenzieri¹, Shaji Kumar¹

Affiliations

¹ Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA.
² Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
³ Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA.

Abstract

We sought to dissect the tumour microenvironment in a small cohort (N = 10) of patients with POEMS at diagnosis and after therapy using mass cytometry. We included 10 MGUS patients as controls. We identified 29 immune cell subsets in the CD45⁺ and CD3⁺ compartments. Double positive T cells and PD-1 positive CD4 T cells were expanded and naïve CD4 T cells were decreased in the bone marrow of patients with newly diagnosed/progressing POEMS. These findings provide evidence for possible antigenic-driven selection as a driver of disease pathogenesis in POEMS.

Keywords: immunohaematology; immunophenotyping; myeloma; tumour immunology.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Female
Flow Cytometry / methods*
Humans
Male
POEMS Syndrome / genetics*
T-Lymphocyte Subsets / immunology*
Tumor Microenvironment

Abstract

Publication types

MeSH terms

Grants and funding