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BMC Genomics. 2020 Feb 17;21(1):165. doi: 10.1186/s12864-020-6570-8.

MicroRNA expression profile analysis in sperm reveals hsa-mir-191 as an auspicious omen of in vitro fertilization.

Author information

1
Shanghai JiAi Genetics & IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, No.588 Fangxie Road, Shanghai, 200011, China.
2
NHC Key Lab. of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research), Hospital of SIPPR, Fudan University, Shanghai, China.
3
NHC Key Lab. of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research), Public School, Fudan University, Shanghai, China.
4
Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China.
5
Department of Obstetrics and Gynecology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
6
NHC Key Lab. of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research), Pharmacy School, Fudan University, No.2140 xietu road, xuhui district, Shanghai, People's Republic of China.
7
NHC Key Lab. of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research), Pharmacy School, Fudan University, No.2140 xietu road, xuhui district, Shanghai, People's Republic of China. ztctianna@163.com.
8
Shanghai JiAi Genetics & IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, No.588 Fangxie Road, Shanghai, 200011, China. steven3019@hotmail.com.
9
Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China. steven3019@hotmail.com.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown.

RESULTS:

In this study, RNA-seq data collected from sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were analyzed for the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER). The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p < 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results.

CONCLUSIONS:

These findings suggest that high hsa-mir-191-5p expression in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).

KEYWORDS:

Embryonic development; Hsa-mir-191; Hsa-mir-34c; RNA-Seq; miRNA

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