Format

Send to

Choose Destination
Curr Neurovasc Res. 2020 Feb 13. doi: 10.2174/1567202617666200214103950. [Epub ahead of print]

Mesenchymal stem cell-derived exosomes rescue oxygen-glucose deprivation-induced injury in endothelial cells.

Author information

1
Department of Operation Room, Heart Center, the First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000. China.
2
Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080. China.
3
Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, China.
4
Department of Clinical Research Center, the First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China.
5
Department of Cardiothoracic Surgery, the First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China.

Abstract

OBJECTIVE:

The effects of mesenchymal stem cell (MSC)-derived exosomes on brain microvascular endothelial cells under oxygen-glucose deprivation (OGD), which mimics cells in deep hypothermic circulatory arrest (DHCA) in vitro, are yet to be studied.

METHODS:

MSCs were co-cultured with primary rat brain endothelial cells, which were then exposed to OGD. Cell viability, apoptosis, the inflammatory factors (IL-1β, IL-6, and TNF-α), and the activation of inflammation-associated TLR4-mediated pyroptosis and the NF-κB signaling pathway were determined. Furthermore, exosomes derived from MSCs were isolated and incubated with endothelial cells to investigate whether the effect of MSCs is associated with MSC-derived exosomes. Apoptosis, cell viability, and the inflammatory response were also analyzed in OGD-induced endothelial cells incubated with MSC-derived exosomes.

RESULTS:

OGD treatment promoted endothelial cell apoptosis, induced the release of inflammatory factors IL-1β, IL-6, and TNF-α, and inhibited cell viability. Western blot analysis showed that OGD treatment induced TLR4, and NF-κB p65 subunit phosphorylation and caspase-1 upregulation, while co-culture with MSCs could reduce the effect of OGD treatment on endothelial cells. As expected, the effect of MSC-derived exosomes on OGD-treated endothelial cells was similar to that of MSCs. MSC-derived exosomes alleviated the OGD-induced decrease in the viability of endothelial cells, and increased levels of apoptosis, inflammatory factors, and the activation of inflammatory and inflammatory focal pathways.

CONCLUSION:

Both MSCs and MSC-derived exosomes attenuated OGD-induced rat primary brain endothelial cell injury. These findings suggest that at least some of the protective effects of MSCs on endothelial cells are mediated by MSC-derived exosomes.

KEYWORDS:

Brain; Endothelial cells; Exosome; Inflammatory factors; Mesenchymal stem cells; Oxygen-glucose deprivation

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center