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Pediatr Res. 2020 Feb 13. doi: 10.1038/s41390-020-0793-x. [Epub ahead of print]

Transcriptome analysis reveals dysregulation of genes involved in oxidative phosphorylation in a murine model of retinopathy of prematurity.

Author information

1
Department of Paediatrics, Jagiellonian University Medical College, Krakow, Poland.
2
Department of Medical Genetics, Jagiellonian University Medical College, Krakow, Poland.
3
Department of Paediatric Research, Oslo University Hospital Rikshospitalet, Oslo, Norway.
4
University of Oslo, Oslo, Norway.
5
Department of Paediatrics, Jagiellonian University Medical College, Krakow, Poland. przemko.kwinta@uj.edu.pl.

Abstract

BACKGROUND:

Retinal gene expression pattern is severely altered after exposition to hyperoxia in mice with oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity. Gene ontology and signaling pathway analyses may add new insights into a better understanding of the pathogenesis of this disease.

METHODS:

Seven-day-old C57BL/6J mice (n = 60) were exposed to 75% oxygen for 5 days and then recovered in room air. The controls (n = 60) were kept in the normoxic conditions. Retinas were harvested immediately following hyperoxia, during the phase of maximal neovascularization, and at the time of neovascularization regression. The retinal RNA samples were evaluated for gene expression using mouse gene expression microarrays. DAVID annotation tools were used for gene ontology and pathway analyses.

RESULTS:

The most significantly enriched signaling pathways during the neovascularization phase of OIR were: focal adhesion; ECM-receptor interaction; PI3K-Akt; oxidative phosphorylation; and Alzheimer's, Parkinson's and Huntington's disease signaling pathways. Genes involved in apoptosis, cell proliferation, cell differentiation, and immune responses were associated with neovascularization regression.

CONCLUSIONS:

Performed analyses revealed the possible involvement of various signaling pathways in OIR pathomechanism, mostly specific to the OIR phase. Dysregulation of genes involved in oxidative phosphorylation may have an impact on neovascularization development.

PMID:
32053824
DOI:
10.1038/s41390-020-0793-x

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