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Microorganisms. 2020 Feb 10;8(2). pii: E237. doi: 10.3390/microorganisms8020237.

Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain Streptomyces Albus Subsp. Chlorinus NRRL B-24108.

Author information

1
Pharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
2
BASF SE, 67056 Ludwigshafen, Germany.
3
Helmholtz-Institut für Pharmazeutische Forschung Saarland, 66123 Saarbrücken, Germany.

Abstract

Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of Streptomyces albus subsp. chlorinus NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies.

KEYWORDS:

Streptomyces albus Del14; albucidin; biosynthetic gene cluster; herbicide; heterologous expression; nucleoside

PMID:
32050690
DOI:
10.3390/microorganisms8020237
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