Format

Send to

Choose Destination
Chem Biol Interact. 2020 Feb 7;318:108978. doi: 10.1016/j.cbi.2020.108978. [Epub ahead of print]

Orientin reduces the inhibitory effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on adipogenic differentiation and insulin signaling pathway in murine 3T3-L1 adipocytes.

Author information

1
Department of Endocrinology & Metabolism, College of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
2
Department of Endocrinology & Metabolism, College of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea; Department of Endocrinology & Metabolism, Kyung Hee University Hospital, Seoul, 02447, Republic of Korea.
3
Department of Medicine, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea; Department of Endocrinology & Metabolism, Kyung Hee University Hospital, Seoul, 02447, Republic of Korea.
4
Department of Endocrinology & Metabolism, College of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea; Department of Endocrinology & Metabolism, Kyung Hee University Hospital, Seoul, 02447, Republic of Korea. Electronic address: cemsuh@gmail.com.

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) accumulates in human body, probably influencing adipocyte differentiation and causing various toxic effects, including wasting syndrome. Recently, orientin, a phenolic compound abundant in natural health products, has been shown to have antioxidant properties. We investigated the protective effects of orientin against TCDD-induced adipocyte dysfunction and its underlying mechanisms. In this study, orientin suppressed TCDD-induced loss of lipid accumulation. Orientin inhibited TCDD-driven decreases in the levels of peroxisome proliferator-activated receptor γ and adiponectin. Orientin also reduced TCDD-induced prostaglandin E2, and cytosolic phospholipase A2α levels, and increased TCDD-inhibited peroxisome proliferator-activated receptor gamma coactivator 1-alpha levels in 3T3-L1 adipocytes. TCDD reduced the levels of insulin receptor substrate 1 and glucose transporter 4, and decreased insulin-stimulated glucose uptake activity; however, orientin diminished these TCDD-induced effects. These results suggest that orientin may have beneficial effects on the prevention of TCDD-induced wasting syndrome and type II diabetes mellitus accompanied by insulin resistance.

KEYWORDS:

2,3,7,8-Tetrachlorodibenzo-p-dioxin; 3T3-L1 adipocyte; Differentiation; Orientin

PMID:
32044341
DOI:
10.1016/j.cbi.2020.108978

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center