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Gene. 2020 May 5;737:144437. doi: 10.1016/j.gene.2020.144437. Epub 2020 Feb 4.

Functional expression of ZNF467 and PCBP2 supports adipogenic lineage commitment in adipose-derived mesenchymal stem cells.

Author information

1
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States.
2
Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN, United States; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, United States.
3
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, United States.
4
Department of Developmental BioEngineering, University of Twente, Enschede, Netherlands.
5
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.
6
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, United States; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, United States. Electronic address: vanwijnen.andre@mayo.edu.
7
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; Department of Medical Genetics, Mayo Clinic, Rochester, MN, United States; Department of Molecular Medicine, Mayo Clinic, Rochester, MN, United States. Electronic address: deyle.david@mayo.edu.

Abstract

Bone marrow-derived mesenchymal stromal/stem cells (BMSCs) have the potential to be employed in many different skeletal therapies. A major limitation to utilizing BMSCs as a therapeutic strategy in human disease and tissue regeneration is the low cell numbers obtained from initial isolation necessitating multiple cell passages that can lead to decreased cell quality. Adipose-derived mesenchymal stromal/stem cells (AMSCs) have been proposed as an alternative cell source for regenerative therapies; however the differentiation capacity of these cells differs from BMSCs. To understand the differences between BMSCs and AMSCs, we compared the global gene expression profiles of BMSCs and AMSCs and identified two genes, PCBP2 and ZNF467 that were differentially expressed between AMSCs and BMSCs. We demonstrate that PCBP2 and ZNF467 impact adipogenic but not osteogenic differentiation, further supporting evidence that AMSCs and BMSCs appear to be adapted to their microenvironment.

KEYWORDS:

Adipogenic differentiation; Adipose-derived mesenchymal stromal/stem cells

PMID:
32032745
DOI:
10.1016/j.gene.2020.144437

Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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