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Indian J Pathol Microbiol. 2020 Jan-Mar;63(1):86-89. doi: 10.4103/IJPM.IJPM_396_19.

Cellular mesenchymal epithelial transition (C-MET) gene copy number variation in gastric adenocarcinoma: A pilot search for new marker for targeted therapy in HER-2/neu resistance.

Author information

1
Department of Pathology, University College of Medical Sciences and GTB Hospital, Delhi, India.
2
Department of Surgical Disciplines, All India Institute of Medical Sciences, Delhi, India.
3
Department of Reproductive Biology, All India Institute of Medical Sciences, Delhi, India.

Abstract

Increasing HER-2/neu resistance in gastric carcinoma has encouraged search for new biomarkers for targeted therapy. Cellular mesenchymal epithelial transition (C-MET) is one such tyrosine kinase inhibitor proposed for personalized salvage treatment. We determined frequency of C-MET gene copy number variation (CNV) by Fluorescent in-situ hybridization (FISH) in gastric adenocarcinoma (GAC) and sought its correlation with conventional clinicopathologic parameters. Dual-coloured FISH was done on 32 GAC cases. C-MET gene and centromere 7 signals were counted under fluorescent microscope and ratio was calculated for each case. Correlation between C-MET CNV and conventional clinic-pathologic parameters was done by Fischer exact test. CNV was identified in the form of amplification and polysomy (3.1% each) and associated with poorer prognostic parameters. Our pilot study highlights limited subset of patients that may benefit from anti-C-MET-targeted therapy and thus could be a novel biomarker for targeted intervention in GAC.

KEYWORDS:

Cellular mesenchymal epithelial transition; HER-2/neu; copy number variation; fluorescent in-situ hybridization; gastric adenocarcinoma

PMID:
32031129
DOI:
10.4103/IJPM.IJPM_396_19
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