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Indian J Pathol Microbiol. 2020 Jan-Mar;63(1):86-89. doi: 10.4103/IJPM.IJPM_396_19.

Cellular mesenchymal epithelial transition (C-MET) gene copy number variation in gastric adenocarcinoma: A pilot search for new marker for targeted therapy in HER-2/neu resistance.

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Department of Pathology, University College of Medical Sciences and GTB Hospital, Delhi, India.
Department of Surgical Disciplines, All India Institute of Medical Sciences, Delhi, India.
Department of Reproductive Biology, All India Institute of Medical Sciences, Delhi, India.


Increasing HER-2/neu resistance in gastric carcinoma has encouraged search for new biomarkers for targeted therapy. Cellular mesenchymal epithelial transition (C-MET) is one such tyrosine kinase inhibitor proposed for personalized salvage treatment. We determined frequency of C-MET gene copy number variation (CNV) by Fluorescent in-situ hybridization (FISH) in gastric adenocarcinoma (GAC) and sought its correlation with conventional clinicopathologic parameters. Dual-coloured FISH was done on 32 GAC cases. C-MET gene and centromere 7 signals were counted under fluorescent microscope and ratio was calculated for each case. Correlation between C-MET CNV and conventional clinic-pathologic parameters was done by Fischer exact test. CNV was identified in the form of amplification and polysomy (3.1% each) and associated with poorer prognostic parameters. Our pilot study highlights limited subset of patients that may benefit from anti-C-MET-targeted therapy and thus could be a novel biomarker for targeted intervention in GAC.


Cellular mesenchymal epithelial transition; HER-2/neu; copy number variation; fluorescent in-situ hybridization; gastric adenocarcinoma

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