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Quintessence Int. 2020;51(3):212-218. doi: 10.3290/j.qi.a43948.

Presence of Trichomonas tenax and Entamoeba gingivalis in peri-implantitis lesions.

Abstract

OBJECTIVE:

The aim was to investigate the presence of Entamoeba gingivalis and Trichomonas tenax in peri-implantitis lesions.

METHOD AND MATERIALS:

A total of 141 individuals were included in this study, of which 40 had clinically healthy implants (group H); the remaining were associated with peri-implantitis (group P). Gingival crevicular fluid was collected using absorbent paper, followed by a dental plaque sample from the peri-implant sulcus/pocket using a titanium curette. The samples were transferred into an Eppendorf tube. Each specimen was divided into two parts. One part was examined under a light microscope at a 10 × and 40 × magnification to detect parasites. The other part was spread on a microscope slide, stained with Giemsa stain, and examined under a microscope at 100 × magnification. Pearson chi-square test was used in the statistical analysis of data, with a significance level of P < .05.

RESULTS:

Although there was no presence of parasite around the healthy implants, two parasites were detected in peri-implantitis lesions. Out of 101 lesions, 31 (30.7%) showed E gingivalis, and 34 (33.6%) presented with T tenax. There was a statistically significant difference between the presence of E gingivalis and demographic data including gender, education status, frequency of dental visits, and brushing frequency. Presence of T tenax in lesions was correlated with frequency of dental visits (P < .05). It was observed that E gingivalis and T tenax were mostly detected in the mandible (P = .004 and .014, respectively) in comparison with the maxilla.

CONCLUSION:

This study showed that peri-implantitis lesions were involved with E gingivalis and T tenax, in contrast to the healthy areas.

KEYWORDS:

Giemsa staining; dental implants; direct microscopy; microbiology; parasite; peri-implantitis

PMID:
32020131
DOI:
10.3290/j.qi.a43948
[Indexed for MEDLINE]

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