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Indian J Med Microbiol. 2019 Jul-Sep;37(3):438-441. doi: 10.4103/ijmm.IJMM_19_267.

Phylogenetic diversity of Orientia tsutsugamushi isolates in patients with scrub typhus in Bengaluru, India.

Author information

1
Department of Microbiology, Bangalore Medical College and Research Institute, Victoria Hospital, Bengaluru, Karnataka, India.
2
Department of Paediatrics, Bangalore Medical College and Research Institute, Victoria Hospital, Bengaluru, Karnataka, India.

Abstract

Scrub typhus has re-emerged as an important cause of acute febrile illness in India. There is a dearth of information on strain diversity of Orientia tsutsugamushi from Karnataka, India, hence the present study sought to address this issue. One hundred clinically suspected cases of scrub typhus/rickettsiosis (as per the DHR-ICMR guidelines) were included. Nested-polymerase chain reaction (PCR) for 56-kDa gene and phylogenetic analysis was performed. PCR was positive in 22 cases and phylogenetic analysis showed the presence of different strains, with predominance of clustering (57%) with Gilliam-type for the first time in Karnataka. Knowledge of genetic diversity has implications in development of diagnostics and vaccine.

KEYWORDS:

Orientia tsutsugamushi; phylogenetic analysis; polymerase chain reaction; scrub typhus

PMID:
32003348
DOI:
10.4103/ijmm.IJMM_19_267
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