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Indian J Med Microbiol. 2019 Jul-Sep;37(3):381-386. doi: 10.4103/ijmm.IJMM_19_44.

Diagnostic performance of mini parasep® solvent-free foecal parasite concentrator for the diagnosis of intestinal parasitic infections.

Author information

1
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
2
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Abstract

Introduction:

Various stool concentration techniques have been used to increase the microscopic detection of parasites. We assessed the enclosed, single-vial, Mini Parasep® technique in comparison to the currently used coprodiagnosis procedures.

Materials and Methods:

A total of 150 stool samples were collected. Samples concentrated by formol-ether method (FEM) and Mini Parasep® SF foecal concentrator and unconcentrated samples were subjected to wet mount, iodine mount microscopy and smear examination by modified acid-fast staining.

Results:

Direct wet mount detected 72 positive samples (48.6%), whereas 77 (51.3%) and 80 (53.3%) samples were detected by FEM and Mini Parasep® SF methods, respectively. The sensitivity of detection of parasites was 98.7%, 95% and 90.1% with Mini Parasep®, FEM and direct microscopy, respectively. A clearer background with less foecal debris and a better yield of Hymenolepis nana, Trichuris trichiura, Entamoeba coli and Giardia lamblia were noted with Mini Parasep®.

Conclusion:

Mini Parasep® SF technique is simple, rapid and less cumbersome than conventional diagnostics, making it suitable for routine use. In addition, it offers higher sensitivity and better background clearance than both direct stool examination and FEM.

KEYWORDS:

Mini Parasep®; parasites; stool concentration

PMID:
32003337
DOI:
10.4103/ijmm.IJMM_19_44
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