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Leuk Res. 1988;12(10):823-31.

Use of the MTT assay for rapid determination of chemosensitivity of human leukemic blast cells.

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Department of Oncology, Queen's University, Kingston, Ontario, Canada.


A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtitre plate spectrophotometer. In the present study, optimal conditions for chemosensitivity testing of human leukemia samples were determined, and the relative chemosensitivity of five patient samples was tested.

[Indexed for MEDLINE]

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