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Bioimpacts. 2020;10(1):37-43. doi: 10.15171/bi.2020.05. Epub 2019 Nov 2.

Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells.

Author information

1
Institute of Materials and Biomaterials, Tehran, Iran.
2
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
4
Stem Cell Technology Research Center, Tehran, Iran.
5
Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
6
Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays. Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.

KEYWORDS:

Calcium deposition; Chondrogenesis; Mesenchymal stem cells; Platelet rich plasma; Transforming growth factor-beta

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