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Nat Commun. 2020 Jan 27;11(1):524. doi: 10.1038/s41467-019-13939-z.

Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity.

Author information

1
Department of Molecular Genetics & Microbiology, Duke University Medical School, Durham, NC, 27701, USA.
2
Department of Neurosurgery, Duke University Medical School, Durham, NC, 27701, USA.
3
Department of Pathology, Duke University Medical School, Durham, NC, 27701, USA.
4
Parker Institute for Cancer Immunotherapy, University of California at San Francisco, San Francisco, CA, 94129, USA.
5
Department of Neurological Surgery, University of California at San Francisco, San Francisco, CA, 94129, USA.
6
Department of Surgery, Duke University Medical School, Durham, NC, 27701, USA.
7
Department of Molecular Genetics & Microbiology, Duke University Medical School, Durham, NC, 27701, USA. grome001@mc.duke.edu.
8
Department of Neurosurgery, Duke University Medical School, Durham, NC, 27701, USA. grome001@mc.duke.edu.

Abstract

Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of CD8 T cell immunity; elicit Th1-promoting inflammation; and lack interference with innate or adaptive immunity. However, notorious genetic instability and underlying neuropathogenicity has hampered poliovirus-based vector applications. Here we devised a strategy based on the polio:rhinovirus chimera PVSRIPO, devoid of viral neuropathogenicity after intracerebral inoculation in human subjects, for stable expression of exogenous antigens. PVSRIPO vectors infect, activate, and induce epitope presentation in DCs in vitro; they recruit and activate DCs with Th1-dominant cytokine profiles at the injection site in vivo. They efficiently prime tumor antigen-specific CD8 T cells in vivo, induce CD8 T cell migration to the tumor site, delay tumor growth and enhance survival in murine tumor models.

PMID:
31988324
PMCID:
PMC6985231
DOI:
10.1038/s41467-019-13939-z
[Indexed for MEDLINE]
Free PMC Article

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