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J BUON. 2019 Nov-Dec;24(6):2280-2286.

LncRNA-ATB promotes apoptosis of non-small cell lung cancer cells through MiR-200a/β-Catenin.

Author information

1
Department of Respiratory Medicine, Yidu Central Hospital of Weifang, Qingzhou, 262500, P.R. China.

Abstract

PURPOSE:

Long non-coding RNAs (lncRNAs) play important roles in cancer, but the effects of lncRNA-ATB on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells remain unclear. Therefore, the present study was conducted to explore the role of this lnc-RNA in NSCLC.

METHODS:

The NSCLC NCI-H838 cell line cultured in vitro were used as the objects of study. First, the expressions of lncRNA-ATB, miR-200a and β-catenin in cells were detected. Then, the expression of lncRNA-ATB was knocked down using siRNA, and the effects of low expression of lncRNA-ATB on miR-200a/β-catenin pathway and apoptosis were studied.

RESULTS:

Compared with normal lung epithelial cells BEAS-2B, NCI-H838 cells had a significantly increased level of lncRNA-ATB (p<0.01), a significantly decreased level of miR-200a (p<0.01) and also a significantly increased level of β-catenin (p<0.01). After knockdown of lncRNA-ATB using si-ATB, the expression level of miR-200a was significantly increased, while that of β-catenin was significantly decreased. Besides, si-ATB remarkably increased the expression of Bcl-2 (p<0.01), and reduced the expressions of cleaved-caspase3 and Cytochrome C (p<0.01) and the apoptosis. In addition, the miR-200a mimic lowered obviously the expression of β-catenin (p<0.05) and reduced apoptosis.

CONCLUSIONS:

This study suggests that lncRNA-ATB promotes the apoptosis of NSCLC cells through inhibiting the expression of miR-200a and reversely promoting the expression of β-catenin.

PMID:
31983095
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