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J Dairy Sci. 2020 Mar;103(3):2077-2088. doi: 10.3168/jds.2019-17512. Epub 2020 Jan 21.

Effects of the vat pasteurization process and refrigerated storage on the bovine milk metabolome.

Author information

1
Department of Chemistry, University of Otago, Dunedin, New Zealand 9016; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China 100081.
2
Department of Food Science, University of Otago, Dunedin, New Zealand 9016.
3
Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China 100081.
4
Department of Chemistry, University of Otago, Dunedin, New Zealand 9016.
5
Department of Chemistry, University of Otago, Dunedin, New Zealand 9016. Electronic address: russell.frew@otago.ac.nz.

Abstract

This study is the first to investigate the evolution of cow milk metabolites throughout the vat pasteurization process and storage using untargeted metabolomics based on a multiplatform approach. Nuclear magnetic resonance and ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry were used for fingerprinting water-soluble nutritional compounds, and headspace gas chromatography-mass spectrometry was used to fingerprint the volatile organic compounds. This study demonstrated that vat pasteurization was an efficient and mild means of milk preservation resulting in only minor changes to the metabolites. The pasteurized milk samples exhibited a stable metabolome during the first 8 d of refrigerated storage. However, at the latter stage of storage, the concentrations of pantothenic acid and butyrylcarnitine decreased, whereas some fatty acids, organic acids, α-AA, peptides, and ketones increased. These selected metabolites that changed during milk storage could be used as potential biomarkers to track the storage conditions of pasteurized cow milk.

KEYWORDS:

pasteurization process; shelf-life study; untargeted metabolomics

PMID:
31980231
DOI:
10.3168/jds.2019-17512

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