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Methods Mol Biol. 2020;2122:113-126. doi: 10.1007/978-1-0716-0342-0_9.

Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos.

Author information

1
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria.
2
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria. michael.nodine@gmi.oeaw.ac.at.

Abstract

Genome-wide characterization of RNA populations in early flowering plant embryos can yield insights into the gene regulatory processes functioning during this formative phase of development. However, early embryonic transcriptomes are technically challenging to profile because of the low amount of RNA obtainable and potential RNA contamination from surrounding nonembryonic tissues. Here we provide a detailed protocol for collecting early Arabidopsis thaliana (Arabidopsis) embryos, generating mRNA sequencing (mRNA-seq) libraries, and basic data processing and quality controls of the resulting mRNA-seq data.

KEYWORDS:

Arabidopsis; Embryo; RNA-seq; Transcriptome

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