Gastric protective activities of fucoidan from brown alga Kjellmaniella crassifolia through the NF-κB signaling pathway

Int J Biol Macromol. 2020 Apr 15:149:893-900. doi: 10.1016/j.ijbiomac.2020.01.186. Epub 2020 Jan 20.

Abstract

Fucoidan has been reported to have abundant biological activities. The objective of the present study was to detect the protective effects of fucoidan from Kjellmaniella crassifolia (KF) newly cultured in Dalian, North of China on aspirin-induced gastric ulcers of the Wistar rat model. The present study showed that inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-10 were effectively regulated in rats pretreated with KF. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities increased significantly in the KF pretreated groups, while the levels of maleic dialdehyde (MDA) decreased. The findings obtained by RT-PCR and western blotting indicated that KF could suppress aspirin-induced NF-κB activation via stabilization of IκB-α and thereby induced the downregulation of COX-2 and iNOS. It was demonstrated that KF exerted positive gastric protective effects via suppression of the inflammatory response and oxidative stress, and the mechanism of KF appeared to mediate the NF-κB signaling pathway.

Keywords: Fucoidan; Gastric protective activities; Inflammatory cytokines; Kjellmaniella crassifolia; NF-κB.

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Biomarkers
  • Cytokines / blood
  • Cytokines / metabolism
  • Disease Models, Animal
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / metabolism
  • Gastric Mucosa / pathology
  • Gene Expression
  • Inflammation Mediators / metabolism
  • Male
  • NF-kappa B / metabolism*
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects
  • Phaeophyceae / chemistry*
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Protective Agents / chemistry
  • Protective Agents / pharmacology*
  • Rats
  • Signal Transduction / drug effects*
  • Stomach Ulcer / drug therapy
  • Stomach Ulcer / etiology
  • Stomach Ulcer / metabolism
  • Stomach Ulcer / pathology

Substances

  • Antioxidants
  • Biomarkers
  • Cytokines
  • Inflammation Mediators
  • NF-kappa B
  • Polysaccharides
  • Protective Agents
  • fucoidan