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Drug Discov Ther. 2019;13(6):299-305. doi: 10.5582/ddt.2019.01065.

Different activities of antitumor immunomodulators to induce neutrophil adherence response.

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Department of Health and Nutrition, Faculty of Human Science, Tokiwa University, Mito, Ibaraki, Japan.
Teikyo University Institute of Medical Mycology, Tokyo, Japan.
Department of Sport and Medical Science, Faculty of Medical Technology, Teikyo University, Tokyo, Japan.


Functions of neutrophils, major participant in host defense mechanisms, are known to be regulated by various types of immunomodulators. Capacity of immunomodulators which are reported to show antitumor effect in vivo to induce neutorophil adherence response in vitro was investigated. Several bacterial immunomodulators (OK-432, Corynebacterium parvum, B.C.G.) and components of bacteria cell walls (lipopolysaccharide (LPS), lipid A, lipoteicoic acid, N-cell wall skelton (N-CWS), muramyl dipeptide (MDP)) and fungal polysaccharides (lentinan, zymosan A, etc.) were tested. Neutrophils prepared from peripheral blood of healthy men were incubated with each immunomodulator at 37°C for 60 min in 96 well plastic plates, then neutrophils adherent to substratum were stained by crystal violet and their optical density at 570 nm was measured as a parameter of neutrophil adherence. Although purified polysaccharides mainly prepared from fungi did not induce the adherent response, not only bacterial bodies and their components but also tumor necrosis factor-α (TNF-α) clearly induced it. On the base of these results, functional classification and typing of immunomodulators by different activities in neutrophil adherence was discussed.


Immunomodulator; bacterial components; fungal polysaccharide; neutrophil adherence; tumor necrosis factor-α

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