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Curr Biol. 2020 Jan 6. pii: S0960-9822(19)31617-3. doi: 10.1016/j.cub.2019.12.022. [Epub ahead of print]

Non-enzymatic Activity of the α-Tubulin Acetyltransferase αTAT Limits Synaptic Bouton Growth in Neurons.

Author information

1
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.
2
Integrated Program in Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
3
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
4
Department of Biology, University of Washington, Seattle, WA 98195, USA.
5
Department of Biology, Integrative Program for Biological and Genome Sciences, and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
6
Department of Physiology and Biophysics, The University of Washington, Seattle, WA 98195, USA.
7
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: wildonger@wisc.edu.
8
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: klei0091@umn.edu.

Abstract

Neuronal axons terminate as synaptic boutons that form stable yet plastic connections with their targets. Synaptic bouton development relies on an underlying network of both long-lived and dynamic microtubules that provide structural stability for the boutons while also allowing for their growth and remodeling. However, a molecular-scale mechanism that explains how neurons appropriately balance these two microtubule populations remains a mystery. We hypothesized that α-tubulin acetyltransferase (αTAT), which both stabilizes long-lived microtubules against mechanical stress via acetylation and has been implicated in promoting microtubule dynamics, could play a role in this process. Using the Drosophila neuromuscular junction as a model, we found that non-enzymatic dαTAT activity limits the growth of synaptic boutons by affecting dynamic, but not stable, microtubules. Loss of dαTAT results in the formation of ectopic boutons. These ectopic boutons can be similarly suppressed by resupplying enzyme-inactive dαTAT or by treatment with a low concentration of the microtubule-targeting agent vinblastine, which acts to suppress microtubule dynamics. Biophysical reconstitution experiments revealed that non-enzymatic αTAT1 activity destabilizes dynamic microtubules but does not substantially impact the stability of long-lived microtubules. Further, during microtubule growth, non-enzymatic αTAT1 activity results in increasingly extended tip structures, consistent with an increased rate of acceleration of catastrophe frequency with microtubule age, perhaps via tip structure remodeling. Through these mechanisms, αTAT enriches for stable microtubules at the expense of dynamic ones. We propose that the specific suppression of dynamic microtubules by non-enzymatic αTAT activity regulates the remodeling of microtubule networks during synaptic bouton development.

KEYWORDS:

Drosophila; acetylation; microtubule; microtubule aging; neuromuscular junction; neuron; synaptic bouton; αTAT1

PMID:
31928876
DOI:
10.1016/j.cub.2019.12.022

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