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Infect Genet Evol. 2020 Jan 3;80:104172. doi: 10.1016/j.meegid.2020.104172. [Epub ahead of print]

Molecular phylogeny of Megalobatrachonema (Nematoda: Ascaridida), with description of a new species based on morphological and molecular evidence.

Author information

1
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China.
2
University of Koblenz-Landau, Department of Biology, Universitätsstrasse 1, D-56070 Koblenz, Germany.
3
University of Koblenz-Landau, Department of Biology, Universitätsstrasse 1, D-56070 Koblenz, Germany; Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Department XXI (Med. Microbiology), Andernacher Street 100, 56070 Koblenz, Germany.
4
Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Department XXI (Med. Microbiology), Andernacher Street 100, 56070 Koblenz, Germany.
5
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China. Electronic address: liangliangex369@126.com.

Abstract

Species of MegalobatrachonemaYamaguti, 1941 (Ascaridida: Cosmocercoidea) are important nematode parasites in amphibians and reptiles. However, the phylogenetic relationship of its included two subgenera Megalobatrachonema and Chabaudgolvania remains unclear. In the present study, a new species of Megalobatrachonema, M. (Chabaudgolvania) wangi sp. nov., was described based on the specimens collected from the lesser spiny frog Quasipaa exilispinosa (Liu & Hu) (Amphibia: Anura) in China. The ribosomal [large ribosomal DNA (28S) and internal transcribed spacer (ITS1-5.8S-ITS2)] and mitochondrial [12S small subunit ribosomal DNA and cytochrome c oxidase subunit 1 (cox1)] target regions of the new species and M. (Chabaudgolvania) terdentatum, together with the 12S region of M. (Megalobatrachonema) hainanensis, were amplified and sequenced for molecular identification and phylogeny. Moreover, in order to clarify the systematic position of the new species and the phylogenetic relationship of the two subgenera Megalobatrachonema and Chabaudgolvania, phylogenetic analyses based on 28S + ITS1-5.8S-ITS2 + 12S sequence data were performed using maximum likelihood (ML) inference and Bayesian inference (BI). The molecular phylogenetic results conflicted with the current classification and challenged the validity of the subgenus Chabaudgolvania, that should be a synonym of the subgenus Megalobatrachonema. The presence or absence of valves in the oesophageal bulb as a key criterion for delimitation of the two subgenera Megalobatrachonema and Chabaudgolvania seems to be unreliable.

KEYWORDS:

Ascaridida; Cosmocercoidea; Frog; Genetic data; Phylogeny; Systematics

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