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Arch Gynecol Obstet. 2020 Jan 4. doi: 10.1007/s00404-019-05429-w. [Epub ahead of print]

microRNA-141 inhibits TGF-β1-induced epithelial-to-mesenchymal transition through inhibition of the TGF-β1/SMAD2 signalling pathway in endometriosis.

Author information

1
Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, No. 139 Renmin Road, Changsha, 410000, Hunan, P.R. China.
2
Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, No. 139 Renmin Road, Changsha, 410000, Hunan, P.R. China. fxlfxl0510@csu.edu.cn.

Abstract

PURPOSE:

Recent studies have demonstrated the differential expression of micro(mi)RNAs in endometriosis. Previously, we reported the low expression of miR-141 in patients with this disease. Epithelial-to-mesenchymal transition (EMT) and the transforming growth factor-beta1 (TGF-β1)-induced SMAD2 signalling pathway are central to tumour proliferation and invasion. However, the role of miR-141 in regulating the TGF-β1/SMAD2 signalling pathway and the associated EMT to be elucidated.

METHODS:

The levels of TGF-β1/SMAD2 signalling and EMT markers expression in eutopic and ectopic endometria of endometriosis were determined by immunohistochemistry and western blot analyses. MiR-141 expression was analysed by quantitative reverse-transcription polymerase chain reaction. Cellular invasion and proliferation were determined by transwell and CCK-8 assays, respectively. Functional assay of miR-141 was performed using plasmid and shRNA transfection methods.

RESULT:

The presence of miR-141, EMT, and TGF-β1/SMAD2 signalling markers were detected in eutopic and ectopic endometria of endometriosis. TGF-β1-induced EMT in Ishikawa (ISK) cells by activating the SMAD2 signalling pathway, whereas miR-141 inhibited the TGF-β1-induced EMT, proliferation and invasion abilities of these cells.

CONCLUSION:

These data identify miR-141 as a novel driver of EMT in endometriosis, implicates the link between miR-141 and TGF-β1/SMAD2 signalling pathway in the context of endometriosis, and underscore the role of EMT in the development of endometriosis.

KEYWORDS:

EMT; Endometriosis; TGF-β1/SMAD2 signalling pathway; miR-141

PMID:
31903498
DOI:
10.1007/s00404-019-05429-w

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