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Anal Biochem. 1988 Jul;172(1):259-63.

Removal of dodecyl sulfate from protein solution.

Author information

1
Department of Biophysical Chemistry, Kitasato University School of Medicine, Kanagawa, Japan.

Abstract

Sodium dodecyl sulfate is one of the most widely used detergents. But, there is no good method to remove dodecyl sulfate (DS) from a small volume of protein solution. We showed that (a) DS in protein solution was removed by the addition of potassium salts with high recovery of protein, and (b) the method was useful before tryptic digestion of protein.

PMID:
3189769
DOI:
10.1016/0003-2697(88)90440-x
[Indexed for MEDLINE]

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