Format

Send to

Choose Destination
Fungal Biol. 2020 Jan;124(1):54-64. doi: 10.1016/j.funbio.2019.11.003. Epub 2019 Nov 15.

Botrydial confers Botrytis cinerea the ability to antagonize soil and phyllospheric bacteria.

Author information

1
Instituto Tecnológico de Chascomús (INTECH), Universidad Nacional de General San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Chascomús, Argentina. Electronic address: pvignatti@intech.gov.ar.
2
Instituto Tecnológico de Chascomús (INTECH), Universidad Nacional de General San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Chascomús, Argentina. Electronic address: mariaelisa@intech.gov.ar.
3
Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Argentina. Electronic address: ejofre@exa.unrc.edu.ar.
4
Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain; Laboratorio de Investigación en Microbiología, Facultad de Ciencias Básicas y Biomédicas, Universidad Simón Bolívar, Barranquilla, Colombia. Electronic address: hbolivar1@unisimonbolivar.edu.co.
5
Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain. Electronic address: javier.moraga@uca.es.
6
UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France. Electronic address: muriel.viaud@inra.fr.
7
Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain. Electronic address: isidro.gonzalez@uca.es.
8
Instituto Tecnológico de Chascomús (INTECH), Universidad Nacional de General San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Chascomús, Argentina. Electronic address: pieckenstain@intech.gov.ar.

Abstract

The role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus demonstrating the inhibitory role of botrydial. A taxonomic analysis showed that these bacteria corresponded to different Bacillus species (six strains), Pseudomonas yamanorum (two strains) and Erwinia aphidicola (one strain). Inoculation of WT and botrydial non-producing mutants of B. cinerea along with Bacillusamyloliquefaciens strain MEP218 in soil demonstrated that both microorganisms exert reciprocal inhibitory effects; the inhibition caused by B. cinerea being dependent on botrydial production. Moreover, botrydial production was modulated by the presence of B. amyloliquefaciens MEP218 in confrontation assays in vitro. Purified botrydial in turn, inhibited growth of Bacillus strains in vitro and cyclic lipopeptide (surfactin) production by B. amyloliquefaciens MEP218. As a whole, results demonstrate that botrydial confers B. cinerea the ability to inhibit potential biocontrol bacteria of the genus Bacillus. We propose that resistance to botrydial could be used as an additional criterion for the selection of biocontrol agents of plant diseases caused by B. cinerea.

KEYWORDS:

Antibacterial compound; Bacillus spp.; Biocontrol; Cyclic lipopeptides; Gray mold; Phytotoxin

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center