Format

Send to

Choose Destination
Stem Cell Res. 2019 Dec 19;42:101689. doi: 10.1016/j.scr.2019.101689. [Epub ahead of print]

Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing.

Author information

1
Murdoch Children's Research Institute, Australia; Department of Paediatrics, University of Melbourne, Australia; Department of Child Health, Universitas Gadjah Mada, Indonesia.
2
Murdoch Children's Research Institute, Australia; Department of Paediatrics, University of Melbourne, Australia.
3
Murdoch Children's Research Institute, Australia; Department of Paediatrics, University of Melbourne, Australia; Department of Anatomy and Developmental Biology, Monash University, Australia.
4
Murdoch Children's Research Institute, Australia.
5
Murdoch Children's Research Institute, Australia; Department of Paediatrics, University of Melbourne, Australia. Electronic address: john.bateman@mcri.edu.au.

Abstract

To develop an iPSC SOX9 reporter line for monitoring differentiation into SOX9 expressing cells such as chondrocytes, cranial neural crest and Sertoli cells, we used gene editing to introduce sequences encoding the tdTomato fluorescent protein into the SOX9 locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. Endogenous SOX9 expression was undisturbed and the tdTomato fluorescent reporter mirrored SOX9 mRNA expression. This iPSC line will be useful for assessing iPSC differentiation into SOX9-expressing cells and enrichment by cell sorting.

PMID:
31884373
DOI:
10.1016/j.scr.2019.101689
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center