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Biochem J. 1978 Oct 15;176(1):97-102.

The mitochondrial localization of coproporphyrinogen III oxidase.

Abstract

The location of coproporphyrinogen III oxidase in mitochondria was studied in rat liver by using the digitonin method or hypo-osmotic media for fractionation. The enzyme was found in the intermembrane space with a fraction loosely bound to the inner membrane. This fraction was released by washing the inner-membrane-matrix complex with alkaline solutions or solutions of high ionic strength. The enzyme in both fractions had the same Km (0.16 micrometer) for coproporphyrinogen III. When incubation was performed in a medium that avoided destruction of enzyme membrane binding, a dramatic increase in activity was observed after sonication of whole mitochondria or of the inner-membrane-matrix complex.

PMID:
31872
PMCID:
PMC1186208
DOI:
10.1042/bj1760097
[Indexed for MEDLINE]
Free PMC Article

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