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J Struct Biol. 2019 Dec 19:107437. doi: 10.1016/j.jsb.2019.107437. [Epub ahead of print]

Amino and PEG-amino graphene oxide grids enrich and protect samples for high-resolution single particle cryo-electron microscopy.

Author information

1
Department of Biochemistry & Biophysics and the Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143, United States.
2
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, United States.
3
Department of Biochemistry & Biophysics and the Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143, United States. Electronic address: agard@msg.ucsf.edu.

Abstract

Cryo-EM samples prepared using traditional methods often suffer from too few particles, poor particle distribution, strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.

KEYWORDS:

Air-water interface; Amino functionalization; Cryo-EM; Graphene oxide; Sample preparation

PMID:
31866389
DOI:
10.1016/j.jsb.2019.107437

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