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Reprod Biol. 2019 Dec 18. pii: S1642-431X(19)30361-4. doi: 10.1016/j.repbio.2019.12.001. [Epub ahead of print]

Peritoneal fluid from women with endometriosis impairs human spermatozoa functionality.

Author information

1
Universidad de Alicante, Departamento de Biotecnología, Alicante, Spain; FISABIO - Hospital Universitario de San Juan de Alicante, Servicio de Ginecología y Obstetricia, San Juan de Alicante, Spain.
2
Universidad de Alicante, Departamento de Biotecnología, Alicante, Spain; Hospital Universitario San Juan de Alicante, Servicio de Ginecología y Obstetricia, San Juan de Alicante, Spain.
3
Universidad Miguel Hernández de Elche, Medicina clínica, San Juan de Alicante, Spain.
4
Hospital Universitario San Juan de Alicante, Servicio de Ginecología y Obstetricia, San Juan de Alicante, Spain; Universidad Miguel Hernández de Elche, División de Ginecología, San Juan de Alicante, Spain.
5
Universidad de Alicante, Departamento de Biotecnología, Alicante, Spain.
6
Universidad de Alicante, Departamento de Biotecnología, Alicante, Spain; Cátedra Human Fertility, Universidad de Alicante, Alicante, Spain. Electronic address: mjose.gomez@ua.es.

Abstract

The success of mammalian fertilization depends largely on spermatozoa physiological events. However, the manner in which endometriosis influence morpho-functional spermatozoa biomarkers is poorly defined. Here, we studied in vitro the effect of peritoneal fluid (PF) from women with endometriosis (PFE) and non-endometriosis (PFNE) on spermatozoa parameters. This research was prospective and double-blind. A total of 45 PF samples were collected from women with (n = 25) and without endometriosis (n = 20). Semen samples were obtained from normozoospermic donors (n = 15) and cultured with 20 % (v/v) of PF or commercial culture medium (controls) during 0, 24, and 48 h. The outcome measures were spermatozoa/viability, motility, tyrosine phosphorylation (TP) and spontaneous acrosomal reaction. In addition, plasma membrane sugars were characterized by lectins [Aleuria aurantia agglutinin (AAA), Concanavalin A (ConA), Peanut agglutinin (PNA), and Wheat germ agglutinin (WGA)]. After a 24 -h culture, results reported a significant decrease in motility in cells cultured with PFE compared to the control, together with differences in the AAA and WGA-binding sites. Moreover, spermatozoa in contact with PFNE presented a significantly lower level of acrosome spontaneous reaction. At 48 h, no differences were observed in the biomarkers studied between the PFNE and the control, excluding ConA-binding sites. On the other hand, cells cultured with PFE exhibited significantly less motility, TP, and differences in the relocation of spermatozoa surface sugars. Viability was not affected in any culture condition. Overall, the effects of PFE could negatively affect spermatozoa quality, contributing to explain and diagnose the infertility associated to male partners of women with endometriosis.

KEYWORDS:

Endometriosis; Infertility; Peritoneal fluid; Spermatozoa quality

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