Format

Send to

Choose Destination
J Clin Endocrinol Metab. 2019 Dec 20. pii: dgz288. doi: 10.1210/clinem/dgz288. [Epub ahead of print]

Testosterone Increases the Expression and Phosphorylation of AMP kinase α in Men with Hypogonadism and Type 2 Diabetes.

Author information

1
Division of Endocrinology, Diabetes and Metabolism State University of New York at Buffalo, Williamsville NY.
2
Division of Endocrinology, Diabetes and Metabolism, Saint Louis University, St. Louis, MO.

Abstract

CONTEXT:

Adenosine 5'-monophosphate-activated protein kinase-α(AMPKα) is a mediator of exercise induced glucose uptake in skeletal muscle.

OBJECTIVE:

We evaluated whether the expression and phosphorylation of AMPKα are reduced in the skeletal muscle and adipose tissue in patients with hypogonadotropic hypogonadism(HH), and whether testosterone replacement therapy results in their restoration.

DESIGN:

This is a secondary analysis of a previously completed trial that showed an insulin sensitizing effect of testosterone therapy in men with type 2 diabetes and HH.

SETTING:

Clinical Research Center at University.

PATIENTS:

Thirty-two men with HH and 32 eugonadal men were compared at baseline.

INTERVENTIONS:

Men with HH were treated with intramuscular injections of testosterone or placebo every 2 weeks for 22 weeks. Quadriceps muscle biopsies and subcutaneous abdominal fat biopsies were obtained before and after 4-hour euglycemic hyperinsulinemic clamp, prior to and after testosterone or placebo therapy.

OUTCOME MEASURES AND RESULTS:

mRNA expression of AMPKα in hypogonadal men was lower by 37% in adipose tissue and 29% in skeletal muscle, respectively, as compared to eugonadal men, while phosphorylated AMPKα was lower by 22% and 28% respectively. Following testosterone replacement, the expression of AMPKα did not alter in the fasting state but increased markedly by 41% and 46% in adipose tissue and muscle, respectively, after the clamp. In contrast, phosphorylated AMPKα increased by 69% in muscle after testosterone therapy but did not change following the clamp.

CONCLUSIONS:

Testosterone modulates the expression of AMPKα and phosphorylated AMPKα. These effects may contribute to the improved insulin sensitivity following testosterone therapy.

KEYWORDS:

AMP kinase; AMPK; adipose; hypogonadism; muscle; testosterone

PMID:
31858126
DOI:
10.1210/clinem/dgz288

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center