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J Exp Med. 2020 Mar 2;217(3). pii: e20190811. doi: 10.1084/jem.20190811.

Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation.

Author information

1
Medical Research Council Human Immunology Unit, Radcliffe Department of Medicine, Oxford National Institute for Health Research Biomedical Research Centre, Medical Research Council Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
2
Wellcome Sanger Institute, Hinxton, Cambridge, UK.
3
Open Targets, Wellcome Trust Genome Campus, Hinxton, UK.
4
University of Gdańsk, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
5
Centre for Computational Biology, Weatherall Institute of Molecular Medicine, Oxford, UK.
6
Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK.
7
Institute of Cellular Medicine, Newcastle, UK.
8
Department of Plastic and Reconstructive Surgery, John Radcliffe Hospital, Oxford University Hospitals National Health Services Foundation Trust, Oxford, UK.
9
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
10
Theory of Condensed Matter, Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UK.
11
Department of Dermatology and National Institute for Health Research Newcastle Biomedical Research Centre, Newcastle Hospitals National Health Services Foundation Trust, Newcastle upon Tyne, UK.

Abstract

Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly infiltrates human skin wounds and comprises a major DC population. Using single-cell RNA sequencing, we show that these cells are largely activated DCs acquiring features compatible with lymph node homing and antigen presentation, but unexpectedly express both BDCA-2 and CD123, potentially mimicking pDCs. Furthermore, a third BDCA-2-expressing population, Axl+Siglec-6+ DCs (ASDC), was also found to infiltrate human skin during wounding. These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease.

PMID:
31845972
PMCID:
PMC7062525
[Available on 2020-09-02]
DOI:
10.1084/jem.20190811

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