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J Integr Plant Biol. 2019 Dec 12. doi: 10.1111/jipb.12894. [Epub ahead of print]

Proteomic and metabolomic profiling underlines the stage- and time-dependent effects of high temperature on grape berry metabolism.

Author information

1
UMR1287 EGFV, Bordeaux University, INRA, Bordeaux Sciences Agro, ISVV, 33140 Villenave d'Ornon, France.
2
Institut of Biochemistry and Biology, Potsdam University, D-14476 Potsdam, Germany.
3
Bordeaux University, Proteome Platform, Bordeaux Functional Genomic Center, 33076, Bordeaux, France.
4
Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
5
Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
6
UMR1287 EGFV, CNRS, Bordeaux University, INRA, Bordeaux Sciences Agro, ISVV, 33140 Villenave d'Ornon, France.

Abstract

Climate change scenarios predict an increase in mean air temperatures and in the frequency, intensity, and length of extreme temperature events in many wine-growing regions worldwide. Because elevated temperature has detrimental effects on the berry growth and composition, it threatens the economic and environmental sustainability of wine production. Using Cabernet Sauvignon fruit-bearing cuttings, we investigated the effects of high temperature (HT) on grapevine berries through a label-free shotgun proteomic analysis coupled to a complementary metabolomic study. Among the 2279 proteins identified, 592 differentially abundant proteins were found in berries exposed to HT. The gene ontology categories "Stress", "Protein", "Secondary metabolism" and "Cell wall" were predominantly altered under HT. High temperatures strongly impaired carbohydrate and energy metabolism, and the effects depended on the stage of development and duration of treatment. Transcript amounts correlated poorly with protein expression levels in HT berries, highlighting the value of proteomic studies in the context of heat stress. Furthermore, this work reveals that HT alters key proteins driving berry development and ripening. Finally, we provide a list of differentially abundant proteins that can be considered as potential markers for developing or selecting grape varieties that are better adapted to warmer climates or extreme heat waves. This article is protected by copyright. All rights reserved.

KEYWORDS:

Vitis vinifera ; berry; heat stress; primary and secondary metabolisms; proteomic

PMID:
31829525
DOI:
10.1111/jipb.12894

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