Engineered assembly of a protein-cucurbituril biohybrid

Chem Commun (Camb). 2020 Jan 2;56(3):360-363. doi: 10.1039/c9cc07198a.

Abstract

A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Bridged-Ring Compounds / chemistry*
  • Bridged-Ring Compounds / metabolism
  • Imidazoles / chemistry*
  • Imidazoles / metabolism
  • Lectins / chemistry
  • Lectins / genetics
  • Lectins / metabolism
  • Nuclear Magnetic Resonance, Biomolecular
  • Ralstonia solanacearum / metabolism
  • Scattering, Small Angle
  • X-Ray Diffraction

Substances

  • Bacterial Proteins
  • Bridged-Ring Compounds
  • Imidazoles
  • Lectins
  • cucurbit(7)uril